Permeation of active substances deep inside the skin is one of the greatest problems of contemporary cosmetology. Substances with molecules larger than 200 nm (vitamins, hyaluronic acid) cannot permeate through the epidermal layer due to the limited space between epidermal cells. Injections or perforation of the epidermis are the only effective methods for permeation through the epidermal layer for molecules with diameter larger than 200 nm. The BioEvolution method uses the technique of micropuncture (microperforation) of the epidermis. As a result the epidermal structure is not barrier. Active substances permeate through the epidermis and spread within the dermis.